Figure 10: Fasting-induced increase in pCREB expression in AgRP neurons of the ARC.
From: Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake
(a) Representative confocal images of hypothalamic sections obtained from Npy-GFP mice after a 20-h fast. pCREB-expressing cells are visualized by an anti-pCREB antibody (red stain, left panel). AgRP (NPY)-positive neurons are visualized by green GFP fluorescence (centre panel). The right panel represents a merged image indicating that most AgRP neurons are pCREB positive. (b) Images corresponding to the ones shown in a were obtained with freely fed Npy-GFP mice, indicating a striking reduction in pCREB-positive AgRP neurons. (c) Quantification of confocal images. The percentage of AgRP-positive neurons (as assessed by GFP fluorescence) staining positive for pCREB was calculated. Data are expressed as means±s.e.m. (13–17 sections from three different adult male mice per group were analysed). **P<0.01 (Student’s t-test). Scale bar, 20 μm.
