Figure 4: AgRP release is essential for the orexigenic effects observed with GsD-AgRP but not with hM3Dq-AgRP mice.
From: Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake
(a,c) The orexigenic effect triggered by GsD activation in AgRP neurons is abolished in the presence of the Ay mutation (a) or by treatment with an anti-AgRP antibody (c). (b,d) The orexigenic effect caused by activation of hM3Dq signalling in AgRP neurons remains unaffected in the presence of the Ay mutation (b) or by treatment with an anti-AgRP antibody (d). Mice received a single i.c.v. injection of either saline, CNO (0.5 μg) or CNO (0.5 μg) plus an anti-AgRP antibody (1 μg). All experiments were carried out with 10- to 16-week-old male mice. Data are given as means±s.e.m. (n=4–8 mice per group). *P<0.05, **P<0.01, ***P<0.001, as compared with the corresponding saline-injected group (a,b, Student’s t-test; c,d, one-way analysis of variance followed by Dunnett’s post hoc test).
