Figure 6: Activation of GsD expressed by AgRP neurons promotes hypothalamic AgRP release.
From: Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake
(a) CNO (15 μM) treatment of hypothalamic slices prepared from GsD-AgRP mice triggers a significant increase in AgRP release (incubation period: 1 h). CNO-induced AgRP release was normalized to basal AgRP release determined during an initial 1-h incubation period in the presence of medium (aCSF) alone. (b) This stimulatory CNO effect was not observed with hypothalamic slices prepared from mCherry-AgRP control mice. All experiments were carried out with 10- to 16-week-old mice. Data are given as means±s.e.m. (n=11–13 mice per group; **P<0.01; Student’s t-test).
