Figure 8: PACAP promotes AgRP expression in a KLF4-dependent manner.
From: Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake
(a,b) Effect of PACAP1–38 (1 μM) on AgRP (a) and KLF4 (b) mRNA expression in GT1-7 cells. GT1-7 cells were incubated for 2 h at 37 °C with PACAP1–38, either in the absence or presence of H89 (75 μM), a PKA inhibitor. (c,d) Effects of Ken, an inhibitor of KLF4 expression, on PACAP1–38-induced increases in AgRP (c) and KLF4 (d) expression in GT1-7 cells. GT1-7 cells were incubated for 2 h at 37 °C with either PACAP1–38 (1 μM), PACAP1–38 plus Ken (5 μM) or Ken alone (5 μM). Data are given as means±s.e.m. (n=3 or 4). *P<0.05, **P<0.01, ***P<0.001, as compared with the PACAP1–38-treated group (a,b, Student’s t-test; c,d, one-way analysis of variance followed by Dunnett’s post hoc test).
