Figure 3: Effect of infection kinetics on the clonal composition of virus-specific CD4+ T cells. | Nature Communications

Figure 3: Effect of infection kinetics on the clonal composition of virus-specific CD4+ T cells.

From: Stepwise B-cell-dependent expansion of T helper clonotypes diversifies the T-cell response

Figure 3

(a) Frequency of Vα2+ cells within env-reactive donor EF4. 1 CD4+ T cells plotted against copies of F-MLV env DNA in the spleens of recipient mice 35 days after adoptive T-cell transfer and FV infection. Symbols represent individual mice. The mouse represented with an open symbol was excluded from the regression analysis. (b) Copies of F-MLV env DNA in the spleens of recipient mice that were infected as adults with FV or F-MLV-N or as neonates with F-MLV-B. All recipients received EF4.1 CD4+ T cells as adults and analysed at the indicated time point after T-cell transfer. Each symbol is an individual mouse. The dashed line denotes the detection limit. (c) Absolute numbers (top) and Vα composition (bottom) of env-reactive donor EF4.1 CD4+ T cells in the spleens of recipient mice after T-cell adoptive transfer in adult mice either infected with F-MLV-N at the time of T-cell transfer or with F-MLV-B as neonates (n=4–15 mice per time point; P<0.001 between the two types of host on days 14–35, Mann–Whitney rank sum test). Closed symbols are the means (±s.e.m.); open symbols are individual mice; the dashed line represents the frequency of Vα2+ cells in preimmune env-reactive EF4.1 CD4+ T cells.

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