Figure 7: IL-6Rα+IL-7R+ CD4+ T cells are detected at late time points post influenza infection.
From: IL-7 signalling represses Bcl-6 and the TFH gene program
Mice were infected with influenza (PR8) and CD4+CD19−Foxp3− cells were analysed by flow cytometric analysis at the indicated day post infection. (a) Nucleoprotein (NP)-specific cells were isolated and sorted into effector TFH (Eff TFH) and non-TFH (Eff TH) populations. Non-TFH effector cells were then assessed for IL-7R expression and further classified as IL-7R low (IL-7RLO) or IL-7R high (IL-7RHI). (b) IL-6Rα expression of the indicated cell population was measured by flow cytometric analysis (geometric mean fluorescence intensity (geoMFI); mean of n=5±s.d.). (c) Percent NP-specific distribution of Eff TFH, TH IL-7RLO and TH IL-7RHI cells at the indicated time points (Day 7, d9, d15, d30, d60) post influenza infection (mean of n=5±s.d.). **P<0.01 (unpaired Student’s t-test).
