Figure 2: Immunofluorescence staining of hiPSC colonies and their parental cells for endogenous L1 ORF1p expression in HFF-1-derived hiPSCs. | Nature Communications

Figure 2: Immunofluorescence staining of hiPSC colonies and their parental cells for endogenous L1 ORF1p expression in HFF-1-derived hiPSCs.

From: Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells

Figure 2

(a) ORF1p staining indicates activation of endogenous L1 expression after reprogramming of HFF-1 cells into lines hiPS-SB4, hiPS-SB5 and hFF-iPS4. Cells were analysed at passages (p) 23, 16 and 60, respectively. Oct-3/4 staining confirmed the pluripotent status of the analysed stem cell colonies. Mesenchymal stem cell marker CD105 (endoglin) is reported to be expressed in HFF-1 cells but not expressed in pluripotent stem cells. (b) Enlarged areas indicated by boxed dashes in a demonstrate cytoplasmic localization of endogenous L1 ORF1p and its accumulation in foci. Scale bars, 20 μm.

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