Figure 2: Effector Pi04314 interacts with Solanum tuberosum PP1c isoforms in yeast-2-hybrid and immunoprecipitation assays.
From: A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease
(a) Yeast co-expressing the PP1c isoforms with Pi04314 grew on histidine (HIS) medium and yielded b-galactosidase (B-gal) activity, while those co-expressed with the control SFI3 or empty vector (EV) did not. (b) Immunoprecipitation (IP) of protein extracts from agroinfiltrated leaves using GFP–Trap confirmed that cMyc-tagged PP1c isoforms specifically associated with GFP–Pi04314 and not with the GFP–SFI3 control. Expression of constructs in the leaves is indicated by +. Protein size markers are indicated in kDa, and protein loading is indicated by Ponceau stain.
