Figure 8: Meiosis prophase I genes are less stable in Meioc−/− gonads. | Nature Communications

Figure 8: Meiosis prophase I genes are less stable in Meioc−/− gonads.

From: Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts

Figure 8

(a,b) RT–qPCR measurements of MPI transcripts in whole Meioc+/+ and Meioc−/−(a) 14.5-d.p.c. ovaries and 8-d.p.p. testes; (b) Meioc−/− adult testes were compared with Meiob−/− adult testes as a reference. (c) Plot of RT–qPCR data for pre-mRNA and accumulated (mature) mRNA in Meioc+/+ and Meioc−/− fetal ovaries and postnatal testes. Dotted lines, projections of minimal and maximal values. (d) Fold loss of mRNA levels was measured after pulse chase ethynyl uridine incorporation performed in Meioc−/− and Meiob−/− adult testes. (e) Treatment of testes with actinomycin D to inhibit transcription revealed higher meiotic mRNA degradation levels in Meioc−/− gonads. The relative expression of Rad21L after 12 and 24 h of actinomycin D treatment compared with mRNA expression levels without drug treatment is presented. Linear regressions were calculated, and slope coefficients representing degradation rates were compared between Meiob and Meioc mutant gonads. Data represent Rad21L expression following exposure to actinomycin D. For a,b,d, mean±s.e.m., n=3–4; *P<0.05, **P<0.01, ***P<0.001 (Student’s t-test).

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