Figure 7: Phosphorylation of Atg13 disassembles Atg1PC and prevents tethering.
From: The Atg1–kinase complex tethers Atg9-vesicles to initiate autophagy
(a) SEC elution profiles of Atg17TC in the presence of Atg1–Atg13 (red), Atg1–Atg13SD (blue), and Atg1–Atg13+ATP (green). Atg13SD and addition of ATP prevents assembly of Atg1PC. (b) α-myc immunoblot of co-floatation experiments of Atg9–PLs with recombinant myc-tagged Atg17 and Atg17TC without and with Atg1–Atg13 (Atg1PC) or Atg1–Atg13SD (ref. 23). Similar experiments with large unilamellar vesicles (LUVs) lacking Atg9core served as controls. Input corresponds to 10% of total protein used for co-floatation. (c) The hydrodynamic radius of SUVs (white bar) and Atg9–PLs (black bar) with similar samples as shown in (b) obtained from DLS-experiments. Mean values±s.d. of N=5 experiments. (d) Model for Atg1PC function, tethering of Atg9-vesicles, and its regulated assembly from and disassembly into subcomplexes. P indicates phosphorylation of Atg13 under vegetative conditions.
