Figure 1: Two-stage delivery of miRNA from PLGA microspheres immobilized on an NF scaffold.
From: Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects
HPs and miRNA formed polyplexes in water. The HP/miRNA polyplexes were encapsulated into the PLGA microspheres via the double emulsion method, and the PLGA microspheres containing the HP/miRNA polyplexes were then attached on the PLLA NF scaffold. The PLGA microsphere-incorporated PLLA scaffolds were implanted into mice. The HP/miRNA polyplexes released from the PLGA microspheres (on the PLLA scaffold) could be taken into cells through endocytosis. Intracellular release of miRNA in the cytosol after enzymatic polymer degradation allows its regulation of gene expression.
