Figure 4: Tumours derived from transformed BESC and EsoSC pedigrees.
From: Mutational spectrum of Barrett’s stem cells suggests paths to initiation of a precancerous lesion
(a) Schematic of stem cell transformation and xenografting in immunodeficient mice. (b) Dendritic relationship of gene expression profiles of EACs, ESCC and tumours developed in immunodeficient xenografted transformed Barrett’s (BE tumour; n=7) and oesophageal (Eso tumour; n=3) stem cell pedigrees. (c) Correlation cluster analysis of genes expressed in BESC-derived tumours and those expressed in EAC. (d) Box plot of gene expression intensity from arrays for four genes marking tumours arising from xenografted transformed BESCs and EAC and squamous cell carcinomas. The top whisker shows maximum, the boxed zone shows 1st quartile, mediam and 3rd quartile, while the lower whisker shows minimum with outliers indicated (see Methods section). (e) Immunohistochemistry on sections of EAC, tumours derived from transformed BESC pedigrees (BE tumour), ESCC and tumours from transformed oesophageal stem cell pedigrees (Eso tumour). Scale bar, 7 mm.
