Figure 8: Encapsulation of ancestral RuBisCO in carboxysomes of extant cyanobacteria.
From: Biochemical characterization of predicted Precambrian RuBisCO
Fluorescence microscopy of Synechococcus elongatus PCC 7942 mutants transformed with ancestral β-MRCA, α-MRCA and α/β-MRCA RbcL subunits fused to CFP (blue) and carboxysomal subunit, CcmN, fused to YFP (green). Ancestral RbcL colocalize to the carboxysome, based on CcmN-YFP fluorescence. Chlorophyll-a (Chl-a) fluorescence from the thylakoid membrane is shown in red. All the strains exhibit spatially distributed fluorescent puncta typical of carboxysome localization. Scale bars, 1 μm.
