Figure 2: Sse interacts with telomeric proteins.

Immunoprecipitation experiments using an anti-Flag affinity gel or an anti-HA affinity matrix from S2 cell extracts co-expressing HOAP-FLAG and HA-Sse (a), Moi-HA and FLAG-Sse (b), Flag-HipHop and HA-Sse (c), Ver-FLAG and HA-Sse (d), FLAG-Sse and HA-Hp1 (e), and FLAG-Eff and HA-Sse (f). As negative control, immunoprecipitations were performed from extracts expressing only the corresponding tagged telomeric protein. It is noteworthy that, with the exception of Eff, Separase precipitates or is precipitated by all telomeric proteins tested. *An aspecific antibody cross-reaction with the protein marker. Lysate, 10% of total protein extracts.