Figure 5: Colocalization of MukBEF complexes with TopoIV and ter.
From: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
(a) Examples of colocalization of MukBEF with ori1 and ter3 in ΔmatP cells (left). ΔmatP cells have the same growth and cell cycle parameters as MatP+ cells (Supplementary Methods). Histograms show the colocalization between ori1, ter3 and MukB-mYPet in ΔmatP (SN302, right). n>2,700 cells. Wild-type data from Fig. 1 are shown for comparison. (b) Colocalization of MukB-mCherry, ParC-mYPet and ori1 in wild-type (ENOX5.130), ΔmatP (KK56) and MukBEQ (ENOX5.178) cells, or ter3 in wild-type (KK57) and ΔmatP (KK58) strains. n>2,400 cells. The brightest pixel was used to localize ParC-mYPet molecules within the cell4. The data represent the mean (±s.d.) of three independent experiments. The percentage represents colocalization in those cells that have foci. Note that ParC-mYPet MukBEQ-mCherry cells grew poorly. See also Supplementary Figs 7 and 8.
