Figure 6: MukBEF and MatP influence ter segregation.
From: MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation
Time-lapse analysis of the time between replisome (DnaN-mCherry) disappearance and ter3 segregation. ter3 replicates ∼2 min before termination at terC, and DnaN takes ∼5 min before it is unloaded after replication termination33; hence 7 min was added to the measured values to estimate ‘cohesion time’, which we define as the time for decatenation of ter3 after its replication. Wild type, ENOX5.212; ΔmukB, AU2047; ΔmatP, AU2120; ΔmatP ΔmukB, AU2125; ΔmatP pCTD, AU2157. n≥20. See also Methods and Supplementary Fig. 9.
