Figure 1: New lineage identified using metagenomic and single-cell genomic approaches.
From: Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs
Workflow used to (a) identify novel SSU rRNA gene sequences globally, along with (b) single-cell genomics pipeline to screen and sequence single cells isolated from geothermal springs samples. For the three geothermal spring environments, we sequenced 13, 2 and 3 SAGs, respectively. SSU rRNA gene, small-subunit ribosomal gene; MDA, multiple displacement amplification; QC, quality control; SAG, single-amplified genome. The photograph of Jinze Pool, Tengchong, China, was taken from ref. 22.
