Figure 2: Maximum likelihood concatenated protein phylogeny and cell imaging for ‘Ca. Kryptonia.’
From: Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs
(a) Phylogeny was based on concatenation of 56 conserved marker proteins, where at least 10 marker proteins were used to infer SAG phylogenetic placement (with the exception of JGI-22 with only six marker proteins recovered). Bootstrap support values ⩾50% are shown with small circles on nodes with robust phylogenetic support. The FCB superphylum is shown in the grey shaded region. Expanded phylogenetic tree for ‘Ca. Kryptonia’ shows the placement of the proposed four genera represented by GFMs and SAGs, along with the estimated genome completeness shown in parentheses. (b) A ‘Ca. Kryptonia’-specific FISH (fluorescence in situ hybridization) probe was designed and used to visualize cells from Dewar Creek Spring sediment samples. ‘Ca. Kryptonia’ cells hybridizing with the probe are green, while other cells are visualized with 4',6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 5 μm.
