Figure 1: p38γ and p38δ modulate postnatal cardiac hypertrophic growth.
From: p38γ and δ promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation
(a) Immunoblot analysis of p38γ and p38δ activation and expression in heart extracts prepared from wild-type (WT) mice at different ages. (b,c) WT, p38γ−/−, p38δ−/− and p38γ/δ−/− mice were killed at 4, 6, 9 and 11 weeks. (b) Heart-weight-to-tibia-length ratio. (c) Cardiomyocyte cross-sectional area quantified in wheatgerm agglutinin (WGA)-stained hearts. Data are means±s.e.m. (n=5–13). **P<0.01; ***P<0.001 (two-way analysis of variance (ANOVA) coupled to Bonferroni post tests). (d) Top: representative haematoxylin and eosin staining of transverse heart sections from 9-week-old WT, p38γ−/−, p38δ−/− and p38γ/δ−/− mice. Bottom: Representative staining with FITC-WGA (green) in hearts from 9-week-old WT, p38γ−/−, p38δ−/− and p38γ/δ−/− mice. (e) Echocardiography results for 9-week-old WT, p38γ−/−, p38δ−/− and p38γ/δ−/− mice. IVS;d (inter-ventricular septum in diastole); LVPW;d (left ventricle posterior wall in diastole); LV (left ventricle). Data are means±s.e.m. (n=5–13). *P<0.05; **P<0.01; ***P<0.001 (one-way ANOVA coupled to Bonferroni post tests).
