Figure 3: Hearts from p38γ/δ−/− mice show reduced mTOR activation.
From: p38γ and δ promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation
(a,b) Immunoblot analysis of mTOR signalling pathway activity (a) and the activation status of translation factors (b) in heart lysates of 9-week-old WT and p38γ/δ−/− mice. Bar charts show quantification of total protein or vinculin-normalized band intensities (n=4). Data are means±s.e.m. *P<0.05; ***P<0.001 (t-test). (c) In vivo measurement of protein synthesis. Mice were injected intraperitoneally with 0.040 μmol g−1 puromycin dissolved in 100 μl PBS. Exactly 30 min after injection, tissues were extracted and frozen in liquid N2 for immunoblot analysis with anti-puromycin antibody (n=4).
