Figure 2: H. influenzae PL transporter mutants produce excessive OMVs.
From: A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria
(a) OMV preparations derived from equivalent OD490 units of cultured wild-type (Rd KW20), deletion mutants (Rd ΔvacJ and Rd ΔyrbE) and complementation strains (Rd ΔvacJ::vacJ and Rd ΔyrbE::yrbE) were analysed for total protein (Bradford), lipooligosaccharide (Purpald) or OMV specific proteins (Dot blot). Mean values with standard error of the mean (s.e.m.) are shown (n=6 biological replicates). Significant differences between the data sets are marked by asterisks (P<0.05; one-way ANOVA followed by Sidak’s multiple comparison post test). (b) Distributions of OMV sizes produced by the strains were determined by nanoparticle tracking analysis. Mean values with standard deviation (s.d.) of mean and mode OMV diameter sizes within each OMV preparation are shown (n=6 biological replicates). (c) Visualization of density gradient purified OMVs by transmission electron microscopy. Shown are representative micrographs. Scale bars, 100 nm.
