Figure 6: Regulation of vacJ and yrbE expression in E. coli.
From: A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria
(a) OMV preparations derived from equivalent OD600 units of cultured wild-type (Ec BW) and deletion mutant (Ec Δfur) were analysed for total protein (Bradford) and lipooligosaccharide (Purpald). Mean values with s.e.m. are shown (n=6 biological replicates). (b) Distributions of OMV sizes produced by the strains were determined by nanoparticle tracking analysis. Mean values with s.d. of mean and mode OMV diameter sizes within respective OMV preparations are shown (n=6 biological replicates). (c) Relative expressions of vacJ and yrbE in Ec BW and Ec Δfur were determined by qRT-PCR. Horizontal bars highlight the mean of each data set (n=6 biological replicates). Dotted lines indicate a relative gene expression of 1. (a,c) Significant differences between the data sets are marked by asterisks (P<0.05; unpaired t-test).
