Figure 7: OMVs increase the serum resistance of H. influenzae.
From: A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria
Serum bactericidal assays were used to determine the serum resistance of Rd KW20, Rd ΔvacJ, and Rd ΔvacJ::vacJ to normal human serum. Groups tagged with ‘+ Rd-OMVs’, ‘+ ΔvacJ-OMVs’, and ‘+ 2x Rd-OMVs’ indicate assays, where Rd KW20 or Rd ΔvacJ OMV preparations were added to corresponding assays using respective physiological final in vitro concentrations or a twofold higher concentration (2 × Rd-OMVs). For details please see the respective method section (serum bactericidal assay). Horizontal bars highlight the mean percentage of survival of each group (n=5–6 biological replicates). The dotted line indicates 100% survival. Significant differences between the data sets are marked by asterisks (P<0.05; one-way ANOVA followed by Sidak’s multiple comparison post test).
