Figure 3: Accumulation of RAG-induced 53BP1 foci in Rag2c/c XLF−/− B cells. | Nature Communications

Figure 3: Accumulation of RAG-induced 53BP1 foci in Rag2c/c XLF−/− B cells.

From: RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair

Figure 3

(a) Representative 3D projections of 53BP1 immuno-staining conducted on ABLki-treated v-abl pro-B cells. (b) Percentage of v-abl pro-B cells harbouring 1, 2 or >2 53BP1 foci 65 h post-ABLki treatment. Histograms represent means±s.d. from four independent experiments with two independent cell lines for each genotype (see Supplementary Table 1 for details). *0.01≤P<0.05, ***P<0.001. (c) Fluorescent light microscopy images of immuno-3D FISH analysis conducted on ABLki-treated Rag2c/c XLF−/− v-abl pro-B cells. Nuclei were stained with anti-53BP1 antibody (green) and subsequently hybridized with the centromeric Igk BAC probe (Igk C, red). Images show 2 nuclei with coincident 53BP1/Igk signals (see Supplementary Fig. 4 for details).

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