Figure 6: Aberrant V(D)J recombination leads to genomic instability in Rag2c/c XLF−/− p53−/− B cells.
From: RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair
(a–c) Genomic instability at the Igk locus in v-abl pro-B cell lines. (a) Schematic representation of the Igk locus, with positions of the BACs used for generation of DNA FISH probes indicated. (b) Representative metaphase from Rag2c/c XLF−/− p53−/− v-abl pro-B cell lines using the Igk C BAC probe (red) combined with Igk V BAC probe (green) and chromosome 6 paint (white). Yellow arrowheads point to broken or translocated chromosome 6. (c) Percentage of aberrant metaphases from v-abl pro-B cell lines of the indicated genotype harbouring chromosomes breaks (white) or translocations (black) involving the Igk locus. Histograms represent means+s.e.m. of two to three independent cell lines (see also Supplementary Table 3). ***P<0.001. (d–f) Genomic instability at the Igh locus in primary pro-B cells. (d) Schematic representation of the Igh locus, with positions of the BACs used for generation of DNA FISH probes indicated. (e) Representative metaphase from Rag2c/c XLF−/− p53−/− pro-B cells using the Igh C BAC probe (red) combined with Igh V BAC probe (green) and chromosome 12 paint (white). Yellow arrowheads point to broken or translocated chromosome 12. (f) Percentage of aberrant metaphases from pro-B cells of the indicated genotype harbouring chromosomes breaks (white) or translocations (black) involving the Igh locus. Histograms represent means±s.e.m. of at least three independent experiments (See also Supplementary Table 4). ***P<0.001.
