Figure 1: HIF-2α suppresses tumour growth in UPS.
From: Epigenetic re-expression of HIF-2α suppresses soft tissue sarcoma growth
(a) Tumour latency of LSL-KrasG12D/+; Trp53fl/fl (KP, n=16) and LSL-KrasG12D/+; Trp53fl/fl; Epas1fl/fl (KPH2, n=19) mice, shown as days post-injection of Ad-Cre virus. The P-value was calculated using a log-rank (Mantel-Cox) test. (b) Left: a cohort of KP (n=7) and KPH2 (n=9) mice were killed 7 weeks post-Ad-Cre virus injection. Representative images of the hind limb where Ad-Cre was injected in each cohort are shown. Right: weight of KP and KPH2 tumours at 7 weeks post-Ad-Cre injection (grams; error bars are±s.e.m.). *P<0.05. (c) Immunohistochemical staining of BrdU in KP (n=5) and KPH2 (n=5) tumours. Left: images representative of KP and KPH2 cohort. Right: quantification of percentage of BrdU+ cells in KP and KPH2 tumours, over 10 high power fields (HPF) per sample (error bars are ±s.e.m.). *P<0.05. Scale bar, 25 μm. (d) Left: representative images of the hind limb of KP (n=5) and LSL-KrasG12D/+; Trp53fl/fl; Arntfl/fl KPA (n=9) mice 7 weeks post-Ad-Cre virus injection. Right: weights of KP and KPA tumours 7 weeks post-Ad-Cre virus injection (grams; error bars are±s.e.m.). *P<0.05. P-values were calculated from a two-tailed Student’s t-test for parts b–d.
