Figure 3: Functional reactivity of atypical TRBV25-1− NKT cell lines to CD1d–lipid Ag.
From: Atypical natural killer T-cell receptor recognition of CD1d–lipid antigens
(a) Histograms depicting CD69 expression on gated Jurkat T-cells lines transduced with the 9C1, 9B1, 9B2, 9B3, NKT15 or control pHLA-specific TCRs, after overnight in vitro co-culture with either WT (CD1d−), CD1d-intermediate (CD1dint) or CD1d-high (CD1dhi)-expressing C1R APCs (left-hand columns), or (b) with CD1dint C1R APCs plus graded concentrations of α-GalCer (C26:0) (right-hand columns). Numbers in each histogram represent CD69 mean fluorescence intensity (MFI). Data in a (three left-hand columns) are representative of two separate experiments; data in b (right-hand columns) are each representative of one experiment.
