Table 2 Affinity measurements of 9C1 and 9B2 TCR mutants to CD1d–α-GalCer.

From: Atypical natural killer T-cell receptor recognition of CD1d–lipid antigens

TCR

K D (μM)

9C1 WT

4

9C1 Tyr31α-Ala

>200

9C1 Tyr31α-Phe

1.9

9C1 Gln112α-Ala

200

9C1 Ser31β-Ala

4.4

9C1 Tyr55β-Ala

180

9C1 Tyr55β-Phe

12

9C1 Gln57β-Ala

17

9C1 Arg109β-Ala

12

9C1 Leu111β-Ala

>200

9B2 WT

4.3

9B2 Gln112β-Ala

7.9

  1. TCR, T-cell receptor.
  2. Binding of soluble 9C1 and 9B2 mutants to CD1d–α-GalCer, as determined by surface plasmon resonance. Dissociation constant (KD) values for 9C1 WT, Tyr31α-Ala, Gln112α-Ala, Ser31β-Ala, Arg109β-Ala, Leu111β-Ala and 9B2 WT represent the mean of two independent experiments, and 9C1 Tyr31α-Phe, Tyr55β-Ala, Tyr55β-Phe, Gln57β-Ala and 9B2 Gln112β-Ala are derived from a single experiment.
Back to article page