Figure 2: Immunization with MOG in IFA induces increased numbers of Tfr cells that are Neuropilin-1^lo.

Dko mice were immunized with MOG35-55 emulsified in CFA or IFA. (a) Absolute numbers of total MOG-specific Tfh cells and Tfr cells of Dko mice immunized with CFA (open circle, as in Fig. 1c) or IFA (solid circle) at indicated time in dLN. (b) Neuropilin-1^lo expression among MOG-specific Tfr cells 7 days after immunization of WT and Dko mice with MOG35-55 emulsified in CFA (white circles) or IFA (black circles). Data shown are from a single experiment (five mice per group) and are representative of three independent experiments. (c) CD138 and B220 staining of CD3⁻ CD138⁺ or B220⁺ dLN cells (see Supplementary Fig. 4) from WT and Dko mice 12 days after immunization with MOG in IFA or CFA. (d) CD95 and Bcl6 staining of CD138⁻ B220⁺ cells. (e) Frequencies of total plasma cells and GC B cells of WT and Dko mice immunized with IFA (solid circle) and CFA (open circle) in dLN 12 days after MOG immunization. (f) Quantity of MOG-specific Ig in the sera of immunized WT and Dko mice estimated using ELISA 12 days after MOG immunization. Data shown are from a single experiment with five mice per group and are representative of two independent experiments. Data were analysed using the non-parametric Mann–Whitney test. ns, not significant, *P<0.05, **P<0.01, mean±s.e.m.