Figure 8: Association of ARHGAP33 with schizophrenia.
(a,b) Quantitative RT–PCR analysis of ARHGAP33 and SORT1 expression in immortalized lymphocytes from schizophrenia patients (schizo.) and age- and sex-matched controls. Significant reductions are observed in ARHGAP33 (each n=45, U=699, P=0.011, Mann–Whitney U-test; a) and SORT1 (each n=45; U=683, P=7.8 × 10−3, Mann–Whitney U-test; b) in schizophrenia patients. The expression levels of these genes were normalized to GAPDH mRNA. Bars show median values. (c) Linkage disequilibrium of ARHGAP33 in the HapMap JPT. Each diamond represents the correlation (r2) between each pair of SNPs, with darker shades representing stronger linkage disequilibrium, as obtained from the HapMap JST samples. (d) The locations of the SNPs analysed in this study. (e,f) Impact of the risk-T-allele on grey matter volume of the left middle temporal gyrus in schizophrenia patients. A significant cluster of the genotype effect in the left middle temporal gyrus is observed in schizophrenia patients, which is shown as cross-hairline (uncorrected P<0.001, cluster size >100; e). Relative grey matter volumes extracted from the left middle temporal gyrus (F1,122=13.5, P=3.6 × 10−4, ANOVA), the right medial frontal gyrus (F1,122=13.5, P=8.2 × 10−4, ANOVA) and the right inferior temporal gyrus (F1,122=13.1, P=4.4 × 10−4, ANOVA; f). *P<0.05. Data are expressed as the mean±s.e.m.
