Figure 3: The depletion of NuSAP or Kid attenuates the amplitude and velocity of centromere movements.

(a) Representative images of the 3D view using xz and yz projections in the control, NuSAP- or Kid-depleted synchronized metaphase mCherry-H2B HeLa cells. Centromeres were marked with GFP-CENPA and centrosomes with GFP-centrin. Scale bar, 5 μm. (b) Representative images of 3D centromere tracks, colour-coded for time and velocity. (c,d) Bar chart and histogram representing the average (c) and the distribution (d) of the amplitude of centromere oscillation in the control, NuSAP- or Kid-depleted metaphase cells. Error bars represent +s.e.m. *P<0.001, by t-test. (e,f) Bar chart and the histogram representing the average (e) and the distribution (f) of the centre velocity. Error bars represent +s.e.m. *P<0.001, by t-test. (g) Representative velocity (solid black line) and ICD (solid grey line) versus time plots of the projected centre in a sister centromere pair in control, NuSAP- and Kid-depleted metaphase cells. The sign of the velocity indicates the direction of the centromere movement. The average of the ICD (horizontal) and the minimum (horizontal) and maximum velocities (vertical) are indicated by dashed lines.