Figure 4: NuSAP and Kid regulate centromere movement at interpolar microtubules.
From: NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force
(a) Representative images of the 3D view using xz and yz projections in Nuf2-, NuSAP/Nuf2- or Kid/Nuf2-depleted synchronized metaphase HeLa cells stably expressing mCherry-H2B. Centromeres were marked with GFP-CENPA and centrosomes with GFP-centrin. Scale bar, 5 μm. (b) Representative images of the 3D centromere tracks colour-coded for time and velocity. (c,d) Bar chart and histogram representing the average (c) and the distribution (d) of the amplitude of centromere oscillation in Nuf2-, NuSAP/Nuf2- or Kid/Nuf2-depleted metaphase cells. Error bars represent +s.e.m. *P<0.001, by t-test. (e,f) Bar chart and histogram representing the average (e) and the distribution (f) of the centre velocity. Error bars represent +s.e.m. *P<0.001 by t-test. (g) Representative velocity (black line) and ICD (grey line) versus time plots of the projected centre in a sister centromere pair in Nuf2-, NuSAP/Nuf2- or Kid/Nuf2-depleted metaphase cells. The sign of the velocity indicates the direction of centromere movement. The average of the ICD (horizontal) and the minimum (horizontal) and maximum velocities (vertical) are indicated by the dashed lines.
