Figure 5: The effects of NuSAP and Kid depletion on the amplitude and velocity of centromere oscillation in monopolar cells are correlated.

(a) Representative images of the 3D view using xz and yz projections in the control, NuSAP- or Kid-depleted monastrol-treated monopolar HeLa cells stably expressing mCherry-H2B. Centromeres were marked with GFP-CENPA and centrosomes with GFP-centrin. Scale bar, 5 μm. (b) Representative images of the 3D centromere tracks colour-coded for time and velocity. (c,d) Bar chart and histogram representing the average (c) and the distribution (d) of the amplitude of centromere oscillation in the control, NuSAP- or Kid-depleted monopolar cells. Error bars represent +s.e.m. *P<0.001, by t-test. (e,f) Bar chart and histogram representing the average (e) and the distribution (f) of the centre velocity. Error bars represent +s.e.m. *P<0.001, by t-test. (g) Representative velocity (black line) and ICD (grey line) versus time plots of the projected centre in a sister centromere pair in the control, NuSAP- or Kid-depleted monopolar cells. The sign of the velocity indicates the direction of centromere movement. The average of the ICD (horizontal) and the minimum (horizontal) and maximum velocities (vertical) are indicated by the dashed lines.