Figure 4: Accumulation of glycerol phosphate and rapid ATP depletion in PI-treated M. tuberculosis.

(a) MIC50 values of glycerol phosphate (black squares), DHAP (blue triangles) and methylglyoxal (red diamonds) were determined for M. tuberculosis H37Rv. M. tuberculosis H37Rv was exposed to 1 and 2 in the presence of glycerol for 24 h. The relative abundances of glycerol phosphate (b) and of pyruvate (c) are shown. M. tuberculosis H37Rv was exposed to 1 and 2 in the presence of glycerol for 24 h. The intracellular ATP level was quantified (d). Streptomycin and moxifloxacin were used as reference compounds.