Figure 5: Functional analysis of UBXN-3-binding domains. | Nature Communications

Figure 5: Functional analysis of UBXN-3-binding domains.

From: Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression

Figure 5

(a) Schematic illustration of full-length UBXN-3, truncated peptides and FPK-motif mutants used for binding studies. Full-length UBXN-3b consists of 608 amino acids (aa). (b,c) wt or NPL-4-depleted worm lysates were incubated with the indicated, recombinant UBXN-3 variants coupled to Ni-NTA beads and analysed by western blotting. Eluted proteins were detected by immunolabeling of ubiquitin conjugates (FK2), CDT-1 and CDC-48.

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