Figure 3: VCBP-C colocalizes with chitin-rich mucus at the surface of the stomach epithelium near the midgut and is visualized with confocal microscopy. | Nature Communications

Figure 3: VCBP-C colocalizes with chitin-rich mucus at the surface of the stomach epithelium near the midgut and is visualized with confocal microscopy.

From: Gut immunity in a protochordate involves a secreted immunoglobulin-type mediator binding host chitin and bacteria

Figure 3

(a) Chitin staining (arrow) detected by Fc-CBD-C DyLight 488 (green), (b) VCBP-C staining (arrow) detected by Alexa Fluor 594 (red) at the surface mucus as well as within granules of the epithelium, (c) Hoechst staining of DNA and (d) merged (arrow; overlay indicated in yellow). Staining with isotype and secondary antibody controls at varying concentrations was negative. Scale bars, 50 μm. E, epithelium; L, lumen.

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