Figure 3: Light regulation of synthetic transcriptional repressors. | Nature Communications

Figure 3: Light regulation of synthetic transcriptional repressors.

From: Optogenetic control of nuclear protein export

Figure 3

(a) Schematic of used constructs for light control of transcriptional repression. DBD, DNA binding domain; KRAB, Krüppel associated box; LexA TF, synthetic LexA transcription factor; LexA TR-LEXY, LexA repressor fused to mCherry-LEXY; NLS, cMycP1A NLS; VP64, a strong transactivation domain; 2A, T2A peptide. (b) Quantification of relative luciferase activity in HEK 293T cells transiently transfected with the constructs in a and irradiated with blue light pulses for 24 h or incubated in the dark. Positive control, cells co-expressing LexA TF and NLS-mCherry-LEXY. Mean±s.d., n=4 independent experiments; *P<0.05, **P<0.01, ***P<0.001 by Welch’s t-test. NS, not significant.

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