Figure 1: LPA activates Arf6 to promote the invasion of 786-O cells.
From: Lysophosphatidic acid activates Arf6 to promote the mesenchymal malignancy of renal cancer
(a) The expression of Arf6 pathway proteins in ccRCC cell lines, assessed by immunoblotting analysis of total cell lysates using the indicated antibodies. Lysates of MDA-MB-231 cells were included for comparison. β-actin immunoblotting is shown as a control. (b) Matrigel invasion activities of 786-O cells in the presence of various ligands, as indicated. (c) Arf6 activity of 786-O cells on stimulation by LPA. (d) Requirement for Arf6, AMAP1 and EPB41L5 in LPA-induced Matrigel invasion of 786-O cells, as assessed by their gene silencing using specific siRNAs. (b,d) Error bars show the mean±s.e.m., n=3. *P<0.01. Statistical analyses were performed using analysis of variance.
