Figure 1: The sugar preference assay.

(a) Schematic of the experimental set-up used to measure sucrose preference. (b–e) Examples of the distribution of starved (36 h) Canton S flies in the circular arena at various time points (5–60 s) after introduction. A 2-M sucrose solution was used for the sucrose side (lower semicircle). (f–i) Same images as in b–e after applying a suitable threshold with the Fiji software. Identified particles are in black. (j) Starvation dependency of sucrose PI of Canton S flies. A 2-M sucrose solution was used. n=8–12 per starvation interval. (k) Concentration dependency of sucrose PI of Canton S flies. Flies were starved to 20% mortality (average starvation time 40 h). n=11–15 per concentration. Results are means±s.e.m.