Figure 4: Anatomy of atGRNs in the periphery and CNS.

(a) Subtraction of Gr5a-LexA expression from Gr64f-GAL4 with LexAop-GAL80 restricts UAS-effector expression to non-overlapping GRNs. (b–f) Expression pattern of Gr(64f–5a) in the foreleg (b), the fifth tarsal segment (c,d), the GNG (e) and the VNC (f). UAS-mCD8::GFP, orange; Synapsin, blue; scale bars, 40 μm. (b–d) Only 1–2 pairs of atGRNs were labelled in the forelegs. atGRNs have cell bodies in the fifth tarsal segment (arrowheads) and innervate a distinct pair of ventral sensilla in the distal part of the foreleg (arrows). (e) Input to the GNG in Gr(64f–5a) flies derives from the VNC (arrowhead). GFP was not detected in fibres projecting from the labellum. (f) Tarsal fibres project to the VNC and ascend via the cervical connective to the GNG (atGRNs, arrowheads).