Figure 7: stGRNs are required for sugar-dependent turning suppression.

(a–c) Examples of locomotion of (a) Gr5a-GAL4/+, (b) Gr5a-GAL4/UAS-Kir2.1 and (c) UAS-Kir2.1/+ flies during half-second intervals (x=46.4 s in a, 55.5 s in b and 50 s in c). Flies are colour-coded according to time. Scale bars, 3 mm. (d) Average absolute angular velocity of all flies between 30 and 60 s of the experiment in the absence (light grey, n=15–16) or presence (dark grey; n=16–17) of 2 M sucrose. Sucrose significantly reduced angular velocity for genetic controls, but not for the experimental group (Mann–Whitney U-tests; ***P<0.001; not significant P>0.05). (e–g) Examples of locomotion of (e) Gr5a-GAL4/+; tub>GAL80>/+, (f) Gr5a-GAL4/otd-nls-FLPo; tub>GAL80>/UAS-Kir2.1 and (g) otd-nls-FLPo/+; UAS-Kir2.1/+ flies during half-second intervals (x=56 s in e, 51.8 s in f and 31.6 s in g). Flies are colour-coded according to time. Scale bars, 3 mm. (h) Average absolute angular velocity of all flies between 30 and 60 s of the experiment in the absence (light grey, n=13–14) or presence (dark grey; n=13–14) of 2 M sucrose. Sucrose significantly reduced angular velocity for all groups (Mann–Whitney U-tests; ***P<0.001; **P<0.01; *P<0.05). (i–k) Examples of locomotion of (e) Gr64f-GAL4/+, (f) Gr(64f–5a) block and (g) the associated effector line control flies during half-second intervals (x=49.5 s in i, 47.6 s in j and 51.7 s in k). Flies are colour-coded according to time. Scale bars, 3 mm. (l) Average absolute angular velocity of all flies between 30 and 60 s of the experiment in the absence (light grey, n=8–11) or presence (dark grey; n=9–11) of 2 M sucrose. Sucrose significantly reduced angular velocity for all groups (Mann–Whitney U-tests; ***P<0.001; **P<0.01). Results are medians, with the error bars indicating the first/third quartile.