Figure 3: The fusion peptide from IAV hemagglutinin inhibits STING dimerization and TBK1 phosphorylation in response to membrane fusion.
From: Influenza A virus targets a cGAS-independent STING pathway that controls enveloped RNA viruses
(a) iBMDMs expressing GFP-tagged STING were stimulated with dsDNA or with fusogenic liposomes (Lipo, 2 μg ml−1). Cells were pretreated with FP for 15 min or left untreated. After 1 h, cells were fixed and stained for giantin and with DAPI. White scale bar is 5 μm. (b–f) THP1 cells were pretreated with FP (10 μM) as indicated and stimulated for 3 h. Cell lysates were prepared and separated by SDS-PAGE under non-reducing (b,e) or reducing (c,f) conditions and immunoblotted for STING, pTBK1 or β-actin. All panels represent data from one of at least two independent experiments. (d) Total RNA was isolated 4 h after stimulation and levels of ISG54 mRNA were determined by RT–qPCR, normalized to β-actin and shown as means±s.e.m.
