Figure 4: The IAV HA2 FP binds STING.
From: Influenza A virus targets a cGAS-independent STING pathway that controls enveloped RNA viruses
(a) Hemagglutinin (HA)-tagged mSTING was expressed in HEK293T cells, lysates were mixed with biotinylated FP and precipitated with streptavidin beads. Co-precipitated STING was detected by immunoblotting. (b) Graphic presentation of the STING region encompassing the dimerization interphase and the FP-interacting region (FIRe). (c,d) His-tagged hSTING CTD+FIRe hSTING (c,d) or His-tagged pOAS1 (d) were mixed with biotinylated FP and precipitated using streptavidin beads. In c the cellular lysates from HEK293 cells were added as indicated. The precipitates were analysed for hSTING or pOAS1 by immunoblotting for His. (e) Solutions of hSTING CTD+IFRe alone, hSTING CTD+IFRe plus FP, FP alone and pOAS1 plus FP were subjected to analytical SEC. High molecular fractions were separated by SDS-PAGE and silver-stained together with a positive control for FP (Pos FP). All experiments were performed at least two times with similar results.
