Figure 3: Maternal deletion of Ube3a has no apparent effect on intrinsic excitability and inhibitory input onto VTA neurons.

(a) Representative traces and average data showing action potential firing rates to increasing current injections in Ai9-positive VTA neurons in THCRE::Ai9::Ube3am−/p+ and THCRE::Ai9::Ube3am+/p+ mice (n=18 per group, Student’s t-test, P=0.70). (b) Average values of resting membrane potential (Student’s t-test, P=0.46), membrane resistance (P=0.25), maximum instantaneous firing frequency (Student’s t-test, P=0.22), and average action potential peak amplitude (Student’s t-test, P=0.32) of Ai9-positive neurons. (c) Representative traces and average data showing the frequency and amplitude of sIPSCs in Ai9-positive neurons (n=12 for each genotype, Student’s t-test, P=0.42, P=0.24). GABAergic currents were validated by bath application of SR95531. All bars represent mean±s.e.m.