Figure 6: H2A.X expression is correlated with that of the EMT-inducing transcription factors Slug and ZEB1 in human adenocarcinoma.
From: The histone variant H2A.X is a regulator of the epithelial–mesenchymal transition
(a–c) Statistically significant correlation between transcript levels for H2A.X and Slug (a) or ZEB1 (b) in 233 adenocarcinoma samples in the TCGA COAD data set. No significant correlation was found between the level of H2A.X and SNAI1 (c) in the same data set. The P values were calculated using the non-parametric Mann–Whitney t-test. The Log 2 expression level is shown for the indicated genes. (d) Inverse correlation between the transcript levels of H2A.X (top) and Slug (middle) or ZEB1 (bottom) in the colon cancer subpanel of the NCI60. The Z score represents the transcript expression in standard deviation units relative to the gene-specific mean expression level. (e) Invasion rate in parental cells (WT), H2A.X knockout cells (KO) and revertant cells (KO+H2A.X). Data are means±s.d.; n=3. Statistical significance was determined by a two-tailed, unpaired Student’s t-test. (f) Hypothetical model for the role of H2A.X in EMT and metastatic colonization.
