Figure 1: RNA polymerase activity at ambient temperature and in ice. | Nature Communications

Figure 1: RNA polymerase activity at ambient temperature and in ice.

From: Ice as a protocellular medium for RNA replication

Figure 1

(a) Schematic of the polymerase ribozyme. The catalytic and processivity domains of the ribozyme (black) are depicted surrounding the primer-template duplex. The 5′ terminus of this ribozyme variant is designed to hybridize on the RNA template (green) upstream of the primer (orange) (Supplementary Fig. S1). (b) Fluorescent primer extension reactions using a proteinaceous polymerase (T7 RNA polymerase) and the ribozyme RNA polymerase at ambient temperatures (red) and in ice (blue), resolved by denaturing gel electrophoresis. (c) Average primer extension in diluted reactions after 7 days at 17 °C (red circles) or in ice at −7 °C (blue diamonds) relative to undiluted reactions at these temperatures (means ± s.e.m., n=3).

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