Figure 2: Different NLRP3 and NLRC4 expression in lung cells from CF mice. | Nature Communications

Figure 2: Different NLRP3 and NLRC4 expression in lung cells from CF mice.

From: IL-1 receptor antagonist ameliorates inflammasome-dependent inflammation in murine and human cystic fibrosis

Figure 2

C57BL/6 and Cftr−/−mice (n=6 for all groups) were infected intranasally with live A. fumigatus conidia or P. aeruginosa and assessed for (a) NLRP3 and (b) NLRC4 protein expression in the lungs and lung epithelial and myeloid cells (magnified in the insets) by immunohistochemistry. Cell nuclei were counterstained with haematoxylin. Representative images of two independent experiments were acquired with a × 20 and × 60 (inset, using EVOS FL Color Imaging System) objective. Scale bar, 200 μm. Immunofluorescence staining with (c) NLRP3 followed by anti-rabbit TRICT or (d) pNLRC4 and NLRC4 of epithelia cells and macrophages purified from lungs and neutrophils from the peritoneal cavity of C57BL/6 and Cftr−/− uninfected mice exposed in vitro to LPS+ATP, flagellin, A. fumigatus live conidia or P. aeruginosa. Cell nuclei were counterstained blue with DAPI. Images were acquired a high-resolution Microscopy Olympus DP71 using a × 100 objective. Scale bar, 12.5 μm. For number of cells with positive NLRP3 or NLRC4 expression quantification, see Supplementary Fig. 1.

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