Figure 5: NLRC4 produces IL-1Ra. | Nature Communications

Figure 5: NLRC4 produces IL-1Ra.

From: IL-1 receptor antagonist ameliorates inflammasome-dependent inflammation in murine and human cystic fibrosis

Figure 5

C57BL/6, Nlrp3−/−, Nlrc4−/− and Cftr−/− mice (n=6 for all groups) were infected intranasally with live A. fumigatus conidia or P. aeruginosa. NLRP3 gene (a) and protein (b) expression by RT–PCR or immunofluorescence staining (anti-NLRP3 antibody followed by anti-goat TRICT secondary antibody) of lungs at 7 dpi. Cell nuclei were stained blue with DAPI. Representative images of two independent experiments were acquired with a high-resolution Microscopy Olympus DP71 using a × 20 objective. Scale bar, 200 μm, inset 50 μm. IL-1Ra production (quantified by specific ELISA) in (c) supernatants of purified lung epithelial cells exposed to the different stimuli, with and without 2 mM of EDTA for 6 h at 37 °C and (d) lung homogenates at different dpi. Data are representative (immunofluorescence) or pooled from three experiments and presented as mean±s.d. for all bar graphs. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, C57BL/6 versus Nlrp3−/−, Nlrc4−/− and Cftr−/− mice at different dpi. Two-way ANOVA Bonferroni post hoc test. For NLRP3 quantification, see Supplementary Fig. 1.

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