Figure 5: PHF20 is required for the E2-induced expression of endogenous ERα target genes. | Nature Communications

Figure 5: PHF20 is required for the E2-induced expression of endogenous ERα target genes.

From: G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression

Figure 5

(a) Western blot analysis of PHF20 levels in control (shCtrl) and PHF20 knockdown (shPHF20) cells. Tubulin was used as a loading control. (b) PHF20 is required for E2-incuded expression of GREB1 and PR. qPCR analysis of gene expression in control and PHF20 knockdown cells treated with 10 nM of E2 for 3 or 6 h. Error bars indicate the mean ±s.e.m. of three experiments. *P<0.05 (Student’s t-test). (c) Venn diagram of the E2-activated genes in MCF-7 cells that are differentially affected by PHF20 knockdown. Approximately 33% of E2-induced genes require PHF20 for gene activation. P<1e-104 (Fisher’s exact test). (d) Venn diagram showing the E2-activated genes in MCF-7 cells that are differentially affected by G9a knockdown or PHF20 knockdown. About 20% of E2-induced genes require both G9a and PHF20 for gene activation. The P value of the overlap between G9a-dependent and PHF20-dependent E2-activated genes is <1e-300 (Fisher’s exact test). The genes of each group are listed in Supplementary Data 1.

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