Figure 1: Spontaneous lymphangiogenesis in Mmp14−/− mice. | Nature Communications

Figure 1: Spontaneous lymphangiogenesis in Mmp14−/− mice.

From: MT1-MMP sheds LYVE-1 on lymphatic endothelial cells and suppresses VEGF-C production to inhibit lymphangiogenesis

Figure 1

(a) Double immunostaining of corneal lymphatic and blood vessels using LYVE-1 (green)- and CD31(red)-specific antibodies in P18 WT (A) and Mmp14−/− mice (B). Quantification of corneal area covered by LYVE-1+ lymphatic vessels and CD31+ blood vessels was shown in the right panel (**P<0.01, n=5, two-tailed t-test). Scale bars, 200 μm. (b) Corneas from P8 WT (A,C,E) and Mmp14−/− mice (B,D,F) were immunostained with LYVE-1 (green) and CD31 (red). Images with a higher magnification in the white-boxed areas and in the yellow-boxed areas were shown in C,D and E,f, respectively. Scale bars, 200 μm (A, B), 100 μm (C,D) and 25 μm (E,F). (c) Quantifications of vascularization area (left panel) and branching points of each ingrowth lymphatic vessel (right panel) of b (**P<0.01, n=5, two-tailed t-test). (d) Lymphatic vessels of diaphragms from P13 WT (A,B) and Mmp14−/− (C,D) mice were stained with LYVE-1 (green). Scale bars, 400 μm (A,C) and 200 μm(B,D). (e) Relative lymphatic vessel density shown in d (**P<0.01, n=5, two-tailed t-test). Data represent the mean±s.e.m.

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